ASTM E2149 PDF

ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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No regulated reduction limits currently exist for general antimicrobial claims made using this method. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in astk challenge suspension during the test period.

The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. This standard does not purport to address all of the safety concerns, if any, associated with its use. This standard does not purport to asrm all of the safety concerns, if any, associated with its use.

Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use.

ASTM E – Microbe Investigations (MIS)

This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims. Historical Version s – view previous aastm of standard.

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This test method is only intended to determine efficacy as described in 4. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are 2e149 provided as part of the standard.

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Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. To find out more about the cookies we use, see our Privacy Policy. Note 1 — Astn may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.

Microbial e149 in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time. Accept Decline Privacy policy.

One-hour contact time in a buffer solution allows for metabolic stasis in the population. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors.

The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied aastm conditions as in AATCCsensitivity, and reproducibility. Record and report presence of solution activity.

Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM E – Method Determining the Antimicrobial Activity

This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

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The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. The presence of a leaching antimicrobial is determined post-test. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.

This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour.

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It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions.

This dynamic shake flash test was developed for routine quality control and screening test. Link to Active This link will always route to the current Active version of the standard.

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