PDF | The present investigation was carried out to evaluate the antioxidant nature of ethanolic extract of Terminalia arjuna bark (EETA) on. Bioactive Components and Antioxidant Properties of Terminalia arjuna L. Extracts . Shahid Chatha, Shahzad Ali, Department of Applied Chemistry. Barks extracts of four different trees (Azadirachta indica, Terminalia arjuna, Acacia Antioxidant activity (AA) was determined by measuring reducing power, .
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Qasim Ali, Assistant Professor antioxidwnt Botany. Where, A blank is the absorbance of the control reaction mixture, and A sample is the absorbance of the sample.
February 28, Citation: Upon arrival in the laboratory the leaves and stem bark samples were washed thoroughly with tap water to remove any wastes and dust particles. Many plant based compounds such as ascorbic acid, polyphenols, vitamin E, carotenes, xynthophylls and tannins had been investigated for their antioxidant potential to be used in different food products to improve their shelf life and functional properties [ 6 ].
It can be concluded from the results that arjuna extracts were good source of natural antioxidants. Home Publications Conferences Register Contact. Briefly, 15 gram of ground material was taken in conical flask followed by the addition of mL of each solvent separately. The arjuna extracts are found to be quite effective in preventing the oxidation. Dry matter extracts mg was mixed with 5.
Two different solvent systems aqueous ethanol water: After 5 minutes, 0. The data regarding the total flavonoid contents, 1. After 3 minutes of shaking, the absorbance of mixtures was measured with spectrophotometer U, Hitachi Instruments Inc,Tokyo, Japan at nm taken as peroxide contents. The data regarding the percentage inhibition of peroxidation ot linoleic acid system from arjuna extracts prepared in different solvent systems is presented in Figure 5.
Bioactive Components and Antioxidant Properties of Terminalia arjuna L.Extracts
The methanol can be exploited for antioxodant extraction of effective antioxidant components from the plant material. The results suggested that extracts of Terminalia arjuna L. Total phenolic contents of extracts was determined by Folin- Ciocalteu reagent due to its high specificity for the polyphenolic compounds present in plant extracts as it did not interact with the other phytochemicals due to a complex formation between reducing species and phosphors-molybdic tungstate.
The reducing power of a compound is related to its electron transfering ability and may serve as a significant indicator of trminalia antioxidant activity [ 20 ]. February 18, ; Published Date: Our finding regarding flavonoids in the present antioxifant were comparable with the findings of other researchers [ 16 ] worked on antioxidant activity antioixdant different plants including Acacia nilotica, Terminalia arjuna, Eugenia jambolana and Azadirachta indica using different solvent blends of ethanol, methanol and water.
The antioxidants are molecules capable of decreasing or preventing the oxidation of substrate molecules. The percentage yield of the extracts was calculated by using the following formula.
There is an ongoing quest in progress to search natural antioxidants from plant extracts as a safe alternate of synthetic antioxidants [ 3 ]. The reducing power was found to be increasing by increasing the concentration of extracts. The arjuna atjuna being a good source of natural antioxidants should be further investigated to explore the chemistry of these extract.
The extraction yields of extracts were ranged from 6.
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A raising trend in the yield of antioxidant extracts has been observed with increasing polarity of solvent suggesting the polar natural of antioxidants compounds. Guidelines Upcoming Special Issues. Sample containing synthetic antioxidant, BHT was used as positive ativity.
Reducing power of ethanolic and methanolic extracts of leaves and stem bark of arjuna at different concentrations. Therefore, this project was designed to investigate bioactive components phenolics and flavonoidsfree activitt scavenging property and antioxidant activity of extracts from barks and leaves of arjuna plant indigenous to Pakistan.
Ethanol showed relatively low yields as compared to methanol, however, ethanol is even then preferred solvent to extract antioxidant compounds due to it lower toxicity.
Bioactive Components and Antioxidant Properties of Terminalia arjuna L. Extracts
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Reducing power of extract was determined according to the procedure described previously [ 15 ]. This is an open-access antioxidamt distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. It was observed that the stem bark extract prepared in methanol, exhibited the highest yield The plant is very rich source of natural antioxidants due to which it has been if investigated for its uses in both Ayurvedic and Yunani systems of medicine [ 78 ].
Methanol is generally employed for the extraction of antioxidant components due to their polarity and good solubility with many antioxidant components [ 17 ]. The sample containing butylated hydroxytoulene BHT was used as a positive control. acticity
Our results are comparable with those ranged from Authenticated samples of leaves and bark were separately packed in polythene bags and transferred to the Natural Products Research Laboratory, Government College University Faisalabad. The specimens was further identified and authenticated from Dr.
The absorbance of the resulting solutions and the blank were recorded after 1 hour at room temperature. Activity of extract increases with the increase in concentration of phenolic compounds.