Download Citation on ResearchGate | Antibacterial activity of Peganum harmala | Successive petroleum ether, chloroform and methanol extracts of Peganum. Successive petroleum ether, chloroform and methanol extracts of Peganum harmala seeds were tested (in vitro) for their antibacterial activity. examine the antibacterial activities of Peganum harmala extracts on the Key words: Antimicrobial effect, Biofilm, Peganum harmala, Pathogenic bacteria.

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Sample collection was done in April for leave and stem, in May for flower, in June for seed and in September for root.

In a study, the alcoholic and aqueous extract of P. Beta-carboline and quinoline alkaloids in root cultures and intact plants of Peganum harmala. Reducing this germ in oral cavity can reduce the rate of tooth decays in humans.

Thin Layer Chromatography can justify this difference, because hramala TLC results for seed antiibacterial root extract showed that these extracts contain several bands with different density or even some bands exist in one extract that we have not been able to find in another one.

In general, antibacterial activity of these two extracts was not considerably affected after temperature and pH treatment. So, it is likely that the active phytochemicals, which exist in root extract, interfere with cell-wall petidoglycan synthesis in bacteria more than seed extract and finally resulted in increasing of the access of colistin to the cell membrane, the outcome of this event is increasing the synergistic effect.

Introduction Nowadays, bacterial infections especially those caused by multi-drug resistant MDR bacteria have become one of the great challenges for modern healthcare. This plant has been considered for the treatment of a variety of human ailments, such as lumbago, asthma, colic, jaundice and as a stimulant emmenagogue Bukhari antbacterial al.


Corresponding studies are suggested to investigate the cytotoxicity of this herb on oral epithelial cells for more accurate results. Introduction Dental caries is one of the most common diseases that humans are afflicted with throughout their lives.

In general, the obtained results from TLC revealed that these two parts of P. Also, pH and temperature stability of seed and root extract is important especially for treatment of brucellosis.

This experiment was carried out in duplicate. It has been reported that harmane as a highly aromatic planar alkaloid exerts its antibacterial activity through interchalate with DNA Cowan, [ 12 ]thus, this antibacterial mechanism must be considered for active extract of Peganu. Also, all of the tested bacteria were resistant to colistin. There are various reports that P.

Antimicrobial Activity of Peganum Harmala L. on Streptococcus mutans Compared to % Chlorhexidine

Moreover, MIC values for seed and root extracts against B. National Center for Biotechnology InformationU. The lawn culture was prepared by sterile cotton swab and allowed to remain in contact for 1 min. Active extract was fractioned using Thin Layer Chromatography; also their synergism activity in combination with synthetic antibiotic was evaluated.

This process was repeated for three times. For instance, pH stability is important in the treatment of brucellosis, one of the most important factors for the treatment of brucellosis is that the antibiotic should be able to act under acidic condition pH 5 in phagolysosomes of macrophages because brucellae grow and replicate in this pH value Akova et al.

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Since the ancient time, the idea of finding healing powers in plants has been noteworthy. These species were originally isolated from clinical specimens and identified by standard biochemical reactions based on morphological and biochemical characters according to the methods described in Bergey’s manual for systematic bacteriology Garrity et al.


Study on the effect of Peganum harmala extract on experimental skin wound healing in rat: Antibacterial effect of leaf part was moderate while stem and flower extracts showed relatively poor activity. Also, the retardation factor R f values of the spots were determined. Antileishmanial activity of Peganum harmala extract on the in vitro growth of Leishmania major promastigotes in comparison to a trivalent antimony drug.

Antibacterial activity of the root extract against tested Antibaccterial species and S. Among the evaluated parts of P. Plants produce a vast array of secondary metabolites that in many cases, these substances act as defense mechanisms against predation by herbivores, microorganisms and insects; also similar substances can be produced by plants as a part of their normal growth and development peganuum in response to stress Mirjana et al.

It seems essential to investigate other antimicrobial agents that have inhibitory effect on relevant microorganisms in oral and dental diseases with fewer side effects compared to chlorhexidine.

Efficient in vitro multiplication of Syrian Rue Peganum harmala L. Toxicity of Peganum harmala: More studies concerning about the molecular basis of this interaction must be performed in future.

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