AFLP TEKNII PDF

AFLP TEKNII PDF

Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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Brazilian breeding programs have exploited the genetic diversity of the BR population and demonstrated that high performance cultivars can be obtained from this population Gerage et al. Variability in Chinese sorghums.

We used the median and maximum coefficient of variation values to evaluate the accuracy of the genetic distance estimates because although the mean coefficient of variation is often used in the literature caution is needed when dealing with molecular marker data feknii which there is no assurance that the CVs values are distributed symmetrically.

Even though the CV values were not low enough to indicate a high level of precision the SSR markers produced high, and the RAPD markers moderate, correlations between the genetic distance estimates and hybrid performance and heterosis for the BR intrapopulational teknui.

From the analysis of our data it appears that the maximum CV value appears to be, in most cases, the best guarantee for producing reliable estimates of genetic distance. Comparison of the genetic distances generated by different molecular markers in diversity studies have been reported by several authors Hahn et al. J Genet Breed The SSR markers were promising in terms of the polymorphism geknii information content revealed, but may involve some additional teknui costs associated with primer development.

Barbosa I ; Isaias O. Am J Hum Teknij Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.

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Codominant markers relate each band to an allele, and therefore the boostrap was applied among locus. The principles and practice of statistics in tekniii research, 3 rd edn.

The Plant J 3: Correlation of genetic distance with F 1 grain yield and heterosis. However, in the case of maize, tropical and temperate populations differ from each other because tropical populations usually originate from composites with higher genetic variability and, most of the time, it is difficult to allocate tropical composites to well-defined heterotic groups by phenotypic evaluation.

Our results indicate that AFLP seemed to be the best-suited molecular assay for fingerprinting and assessing genetic relationships among tropical maize inbred lines with high accuracy. The correlation coefficient values between genetic distance and hybrid performance for the four markers assayed were similar to the correlation values between genetic distance and heterosis, not only for the inter or intrapopulation crosses but arlp for all crosses combined.

As expected, the magnitude of the coefficient of variation CV values decreased as the number of polymorphic loci bands evaluated increased. Although similar average genetic distance values were obtained for the BR and BR intrapopulation crosses, the BR crosses afpp the widest range of genetic distances with all of the four different markers assayed; probably because of the broader genetic base of the BR population. To determine the sampling variance of the genetic distances produced by the different molecular data sets we performed bootstrap analysis using a decreasing number of loci for codominant markers or bands for dominant markers.

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

Our results indicate that, apart from the RAPD markers, the other DNA marker afll provided consistent information for diversity studies on tropical maize populations and produced genetic distance estimates which were in good agreement. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

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The choice of the appropriate number of polymorphic loci required for a reliable estimation of genetic distance is influenced by the criteria used, and it appears that the maximum and median CV values are the best choice for afkp the precision of the genetic distance estimates based on molecular marker data sets.

Eighteen S 3 selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using four different marker systems Lanza et al. Freeman and Company, New York, pp As expected, the PIC distributions revealed that, in terms of genetic distance, dominant markers had lower levels of polymorphism as compared to codominant markers.

AFLP also correlates highly with results obtained using the RFLP system and is a fast and reliable system capable of supporting a multiplex approach not requiring previous knowledge of DNA sequencing.

Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

The application of SSR techniques to plants depends on the availability of suitable microsatellite markers, which have been developed for species such as soybean Rongwen et al. Jaccard similarities were calculated using version aaflp. Although this lower sensibility in detecting informative genotypic classes might be associated with the inability to distinguish heterozygotes from homozygotes because of binary scored AFLPs, Gerber et al. VI Isozyme variation among races of maize in Bolivia.

Comparisons with data from RFLPs and pedigree. Bootstrap analysis As expected, the magnitude of the coefficient of variation CV values decreased as the number of polymorphic loci bands evaluated increased. I ; Anete P. The RAPD markers were clearly the most distinct type of marker because the correlation values involving this marker were equal to or lower than 0.